Anaerobic & Controlled Atmosphere Research

Pharmaceutical, biotechnology and
Anaerobic research glove box solution

Adjustable atmosphere environment, clean maintenance, and safe sample operation and observation platform to meet the needs of hypoxic/anaerobic research and biological process development.

  • Precisely control oxygen concentration to meet research needs from hypoxia to anaerobic
  • Controllable temperature and humidity to stably support cell culture and sample activity
  • Clean ventilation structure, easy to clean and disinfect, reducing the risk of contamination
  • Multi-device and data integration to improve research efficiency and traceability

Application boundary description

Sample protectionMaintain a low-oxygen/anaerobic environment to protect samples and intermediate products
Personnel protectionProvide safe protection functions for researchers
Environmental protectionNo exposure to external BMC environment or biological safety cabinet
Typical requirements
Anaerobic/low oxygen atmosphereStably control O₂ content
Temperature and humidity controlMaintain cell/sample viability
Cultivation equipment integrationIncubator, microscope, etc.
Sample transferSecure transfer in/out
Surface cleaning and disinfectionEasy to clean and disinfectable
Positive pressure/negative pressureFlexible control of cabin pressure
HEPA filtrationClean gas circulation
Data recordsReal-time monitoring and tracing
Typical process
Samples and instruments
Preprocessing
Transfer to transition cabin
Atmosphere creation
Cultivation/experimental operations
Observation and recording
Sealed transfer
Cleaning and Disinfection
Environment recovery

Recommendation system

GBP750S-2 single-station single-sided purification glove box

GBP750S-2 single station purification glove box

The sealed box and gas purification cycle provide a stable and clean atmosphere for anaerobic or low-oxygen operations of oxygen- and water-sensitive samples.

  • Water and oxygen can be controlled below 1 ppm
  • Single station single-sided operation structure
  • Supports inert gas circulation purification
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GBV-2 stainless steel vacuum glove box

GBV-2 stainless steel vacuum glove box

800 × 650 × 650 mm sealed working chamber, which uses vacuum evacuation and inert gas backfilling to reduce the contact between the sample and the air.

  • Stainless steel closed operating chamber
  • Vacuum evacuation and inert gas backfill
  • Suitable for sample processing and material preparation
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GBT plexiglass glove box

GBT plexiglass glove box

The transparent and sealed working space is convenient for observation and operation, and can be used for the processing, transfer and teaching experiments of air and humidity sensitive samples.

  • Highly transparent organic glass working chamber
  • Multiple sizes and vacuum options
  • Visual sample processing environment
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Recommended configurations and options

System functions

NameIntroduction
Automatic controlThe program control unit realizes automatic control and detection of equipment operation.
Box manual/automatic gas replacementSupports replacing the cabinet air with inert gas or nitrogen before first use.
Transition cabin manual/automatic gas replacementWhen materials enter the box, the transition cabin can be manually or automatically replaced with gas.
Gas purification controlIt can automatically maintain the set atmosphere conditions in the box, or switch to manual control.
Foot-operated air pressure controlUse the foot controller to control the inflating or pumping of the box and adjust the air pressure of the box.
Purge System RestoreAfter setting the reduction conditions, the system can automatically complete the purification material reduction process.
Online working condition detectionMonitor the working status of components, assist in locating functions and source of component failures.
System parameter settingsRealize corresponding working conditions and function settings by setting system parameters.
Data logging functionRecord equipment operating parameters for traceability and monitoring of experiments or production processes.
Control air circuit backupWhen the control gas source is temporarily cut off or insufficient, the backup control gas can ensure that the equipment continues to work.
Multi-level security systemProvides multi-level safety prompts, alarms and prohibition functions to ensure the safety of personnel and equipment.
Purification cycle obstruction protectionWhen the circulation is blocked, it prompts an alarm and adjusts the wind speed to protect the motor. It also assists in identifying pipe or filter blockages.

System configuration

Configuration and quantityTechnical parametersRemarks
Standard transition cabin 1 pieceDN385 × 588 mmRight side of the cabinet; SUS304 stainless steel, aluminum alloy door cover, built-in movable drawer and door opening assist device.
Tool transition cabin 1 pieceDN150 × 350 mmThe right side of the box; made of SUS304 stainless steel, can be made as required.
1 piece of shelfDouble layerBack-mounted.
Box rack 1 pieceHeight 900 mmEquipped with swivel casters; a purification system is integrated into the right rack.
Transparent visual panelThickness 12 mmDouble-layer laminated tempered glass.
4 glovesThickness 0.4 mmButyl synthetic rubber.
1 power socket220 V, 10 A, 8 holesLocated inside the box, it supplies power to the electrical appliances in the box.
Lighting systemFluorescent tubeProvides lighting inside the box.
3 sets of spare interfacesKF40Gas or liquid access device; SUS304 stainless steel, equipped with quick connector.
1 vacuum pumpD16C, pumping volume 4 L/SEquipped with oil mist filter.
2 HEPA filtersFilter precision 0.3 μmIt is used to filter dust in the box and protect the ventilation pipes from being unobstructed.
2 vacuum gaugesMechanical dial displayMeasure the vacuum degree of the standard transition cabin and tool transition cabin.
1 pressure gaugeMechanical dial displayMeasure the air pressure of the box.
Foot controller 1 pieceDouble pedal typeControl the gas replenishment and emptying of the cabinet.
Control systemColor touch screen: 6 inchesTouch screen and PLC are configurable; support multi-language operation interface switching.
1 pressure sensor-2500 ~ 2500 Pa (relative pressure)Touch screen display.
Moisture analyzer 1 piece0 ~ 1000 ppm, accuracy 0.1 ppmTouch screen display.
Oxygen transmitter 1 piece0 ~ 1000 ppm, accuracy 0.1 ppmTouch screen display.
Gas purification systemSingle purification columnIt has water and oxygen removal functions and is integrated into the cabinet frame.
Organic solvent adsorption system21 LInstalled in the glove box pipeline to absorb solvent gas generated during use and protect purification materials.

Key control objectives reference

Target oxygen concentration
≤ 0.1 ppm
(Typical value)
Temperature and humidity range
15 ~ 35 ℃
20 ~ 60% RH
HEPA rating
H14
(Intake/Exhaust)
Pressure control
+10 ~ +30 Pa
(relatively external)
Data records
≤ 30 times/hour
(adjustable)

Typical application scenarios

Anaerobic culture application scenarios

Anaerobic culture

Provides a stable, anaerobic culture environment for anaerobic microorganisms to ensure the accuracy of bacterial growth and metabolism research.

Hypoxic cell research application scenarios

Hypoxic cell research

Precisely control the hypoxic atmosphere, simulate the physiological/pathological hypoxic microenvironment, and support the study of cell functions and signaling pathways.

Biological sample processing application scenarios

Biological sample processing

Sample preparation, packaging and transfer are performed in a clean, controlled atmosphere to reduce the risk of contamination and oxidation.

Medical environment simulation application scenarios

Medical environment simulation

Simulate the pharmaceutical production and storage environment to evaluate the stability of materials, packaging and preparations under specific atmospheres.

Why choose TENCAN

Customized engineering capabilities

In-depth understanding of process requirements and providing customized engineering services from solution design to implementation.

Device integration experience

Years of experience in glove box and laboratory system integration, the solution is mature and reliable.

Atmosphere control and testing

Core atmosphere control and detection technology, accurate data and stable operation.

Installation training support

The professional engineering team provides atmosphere debugging and operation training to get started quickly.

Global delivery and after-sales

Global delivery network and localized services, timely response and worry-free after-sales.

Product introduction

Design considerations

  • Disinfection methods and materials compatible:Choose materials and components that can withstand disinfectants and high temperatures.
  • Dead corners inside the cabinet:Reasonable rounded corner design and detachable structure avoid cleaning blind spots.
  • Gloves and sealing materials:Focus on long-term sealing performance, chemical resistance and ease of replacement.
  • Exhaust gas treatment:Choose appropriate filtration and treatment methods based on the type of process gas.
  • Sample transfer method:Choose sealed transfer chambers or rapid transfer interfaces to reduce exposure risks.
  • Whether sterility verification is required:Clear the scope and methods of verification (such as culture medium/challenge test, etc.).
  • Whether GMP/pharmacopoeia/biosafety regulations are involved:Plan ahead for compliance design and documentation requirements.

FAQ

Anaerobic/hypoxic systems place more emphasis on settable biological research atmosphere, culture and cleaning processes; high-purity glove boxes usually place more emphasis on extremely low water and oxygen indicators. Actual solutions can be combined according to sample, personnel and environmental protection objectives.
Yes. Through oxygen sensor, gas mixing and automatic air supply/exhaust control, stable control of hypoxic to anaerobic atmosphere can be achieved according to the target range, and data recording can be configured.
Supported. It can integrate temperature and humidity sensing, heating/cooling, humidification/dehumidification and other modules. The specific control range and accuracy need to be determined based on the cultivation process and indoor environment.
Cleaning, filtration, materials, cleaning, disinfection and verification design can be carried out according to the aseptic operation objectives; whether it meets the requirements of specific production regulations should be confirmed according to the GMP, pharmacopoeia and verification specifications applicable to the project.
Positive pressure is usually chosen when sample protection is the main focus; negative pressure or partitioned pressure difference can be used when it is necessary to reduce the risk of harmful substances escaping to the outside. The final determination should be based on the protection objects, gas properties and laboratory facility conditions.
It is recommended to use rounded inner cavities, easy-to-wipe surfaces and removable parts, and choose wiping, spraying or other compliant disinfection methods based on material compatibility. A verification interface can be reserved if necessary.
Solution Consultation

Find the Glove Box Solution That Fits Your Needs

Tell us about your application, chamber size, H2O/O2 specifications, or integration requirements, and our engineers will recommend a suitable configuration.

  • Standard products and customized integration available
  • Your requirement details are kept strictly confidential
Technical consultation specialist
Questions? Contact our technical specialists.